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. 2016 Feb 4;6:20408. doi: 10.1038/srep20408

Figure 2. Depletion of SUN1 or KASH5 affects mouse oocyte maturation.

Figure 2

(A) Western blots analysis of the knockdown of SUN1 and KASH5 proteins in mouse oocytes by SUN1 Morpholino and KASH5 siRNA microinjection. Microinjected oocytes were arrested at GV stages for 24 h in the presence of milrinone, and 150 oocytes for each treatment were collected and analyzed. (B) Oocyte maturation was affected by SUN1 or KASH5 depletion, with most of the oocytes arresting at the GV stage (p < 0.001). (C,D) SUN1 (45.9%) or KASH5 (45.6%) depleted oocytes with first polar body extrusion underwent asymmetric division or produced a larger polar body, while 6% and 5% (p < 0.001) of the control-injected oocytes respectively displayed any abnormalities. Bar = 100 μM. (E,F) Delayed GVBD in KASH5 knockdown oocytes. KASH5 or scrambled siRNA was injected, and GVBD timing was measured by time-lapse microscopy. Bar  = 20 μM. Time course of GVBD in KASH5 knockdown (n = 13) or Control (n = 9) oocytes was observed, and GVBD rates were measured for each time course analysis. Only oocytes that underwent GVBD in the time-lapse microscopy were analyzed.