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. 2016 Feb 4;6:20408. doi: 10.1038/srep20408

Figure 4. SUN1 or KASH5 depletion leads to the abnormal spindle formation and altered F-actin intensity.

Figure 4

(A) Abnormal spindle formation after SUN1 (upper row) or KASH5 (lower row) depletion. Green: α-tubulin; Red: chromatin. Bar = 20 μM. (B) Ratio of polar body extruded oocytes possessing abnormal spindles after SUN1 or KASH5 knockdown. (C) Spindle size quantifications in SUN1 or KASH5-depleted oocytes. Length of spindle (μm) was measured. (D,E) Cytoplasmic F-actin density decreased in SUN1 or KASH5-depleted oocytes. (F) Spindle location after 7 h of meiotic resumptions in SUN1 or KASH5-depleted oocyte. Green: α-tubulin; Red: chromatin.