Figure 1. Venous and capillary endothelium expresses EMCN in vivo.
(a) Tissues were dissected from adult C5BL6 mice, fixed in 4% paraformaldehyde, frozen in optimal cutting temperature (O.C.T.) compound and cut into 10 μm sections. EMCN localized to venules and capillaries of mouse ciliary bodies, and co-localized with CD31+ endothelial cells in the ciliary body. Ciliary processes in the posterior chamber are indicated with arrowheads. Scale bar, 100 μm. (b) In paraffin sections of normal human skin, EMCN (red) localized on the apical surface of venules marked by collagen IV (green). Nuclei were counterstained using DAPI (blue). Scale bar, 50 μm.