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. 2016 Feb 4;6:20309. doi: 10.1038/srep20309

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(A) Confocal fluorescence imaging of PAT14g-GFP in pat14-2 (PAT14g-GFP;pat14-2) stained with FM4-64. Arrows indicate BFA compartments in which both FM4-64 and PAT14-positive TGN/EE accumulates in the center surrounded by PAT14-positive Golgi stacks. Arrowheads indicate ring-shaped PVC/MVB labeled by PAT14 upon wortmannin treatment. (B) PAT14g-GFP was introduced in transgenic plants expressing RFP-fused markers for Golgi (WAVE22R), TGN/EE (HAP13), and PVC/MVB (VSR2). Partial co-localization was observed for all combinations. Bars = 7.5 μm for (A); 5 μm for (B).