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. Author manuscript; available in PMC: 2017 Jun 1.

Published in final edited form as: J Recept Signal Transduct Res. 2015 Sep 29;36(3):261–270. doi: 10.3109/10799893.2015.1091475

Cells were seeded at 60–80% confluence, incubated overnight, then switch to serum free medium for 2–4 hr. Cells were treated with or without (untreated, UT) phenylephrine (PE, 100 μM) for 16hr and either prazosin (Praz, 1 μM) or the glucose transporter inhibitor II (GTI, 1μM). [³H]-2-deoxyglucose (2DG) was added to each well (0.5 μCi/well) and incubated for 10 minutes. The cells were washed, lysed and transferred to a scintillation vial where ³H-2DG uptake was detected using Beckman scintillation counter. *Statistically significant p≤0.05 from untreated control. ^#Statistically significant p≤0.05 from phenylephrine stimulation. N = 5 independent experiments performed in triplicate.

Cells were seeded at 60–80% confluence, incubated overnight, then switch to serum free medium for 2–4 hr. Cells were treated with or without (untreated, UT) phenylephrine (PE, 100 μM) for 16hr and either prazosin (Praz, 1 μM) or the glucose transporter inhibitor II (GTI, 1μM). [³H]-2-deoxyglucose (2DG) was added to each well (0.5 μCi/well) and incubated for 10 minutes. The cells were washed, lysed and transferred to a scintillation vial where ³H-2DG uptake was detected using Beckman scintillation counter. *Statistically significant p≤0.05 from untreated control. ^#Statistically significant p≤0.05 from phenylephrine stimulation. N = 5 independent experiments performed in triplicate.