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. Author manuscript; available in PMC: 2017 Jun 1.
Published in final edited form as: J Recept Signal Transduct Res. 2015 Sep 29;36(3):261–270. doi: 10.3109/10799893.2015.1091475

Figure 4. α1-AR mediated glucose uptake protected against annexin V+ apoptosis in HL-1 cells.

Figure 4

Cells (105) were subjected to normoxic conditions (control) or with low glucose concentrations (Glu−; 1.375mM) with or without α1-AR stimulation (PE; 100μM). α1-AR stimulated cells were incubated with prazosin, Praz (1μM) or the glucose transporter inhibitor II (GTI, 1μM). Cells were suspended in Annexin V binding buffer (10x stock: 0.1M HEPES, pH 7.4; 1.4M NaCl; 25mM CaCl2. 2 μl of FITC-Annexin V (BD Pharmingen, CA), propidium iodide and incubated for 15 min in the dark. Cells were analyzed using a FACSCalibur flow cytometer (Becton Dickinson, San Jose, Ca) following manufacture’s instructions. Raw data were analyzed by CellQuest software (Becton Dickinson). Cells are expressed as percentage of the total number of stained cells counted. *Statistically significant p≤0.05 from control. #Statistically significant p≤0.05 from Glu−. **Statistically significant p≤0.05 from Glu−-PE. N = 7 independent experiments.