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. Author manuscript; available in PMC: 2016 Aug 1.
Published in final edited form as: Cancer Res. 2015 Dec 4;76(3):724–735. doi: 10.1158/0008-5472.CAN-15-0701-T

Figure 4.

Figure 4

Reintroduction of MLK4-WT decreases cell viability. A, Expression of MLK4-WT in HCT15 and HT115 was induced by tetracycline for 1 day. WCL were analyzed by western blot. B, Expression of MLK4-WT in HCT15 and HT115 was induced by tetracycline for 4 days. Viability was determined by MTT assay. Error bars indicate ±SEM from three independent experiments performed in triplicate (n=9). C and D, HCT15 and HT115 were seeded at low density and expression of MLK4 was induced by tetracycline. After 2 weeks cells were stained with crystal violet (C) and results were quantified by absorbance (D). Error bars indicate ±SEM from three independent experiments (n=3). E, Parental HCT15 and HT115 were treated with tetracycline for 4 days. Viability was determined by MTT assay. Error bars indicate ±SEM from three independent experiments performed in triplicate (n=9). F, Parental HCT15 and HT115 were seeded at low density and expression of MLK4 was induced by tetracycline. After 2 weeks cells were stained with crystal violet and results were quantified by absorbance. Error bars indicate ±SEM from three independent experiments (n=3).