FIGURE 5.

GUS histochemical staining and fluorescent quantitative analysis of transgenic tobacco expressing eight truncated promoter of pZmPIS::GUS under normal and salt stress conditions. (A) GUS histochemical staining; (B) GUS fluorescent quantitative analysis. The tobacco plants were incubated in the liquid 1/2 MS medium supplemented with 200 mM NaCl for 1, 3, 6, 12, 24, 48, and 72 h. The plants grown in the liquid 1/2 MS medium were treated as control. Values represent the mean ± standard deviation from three independent transgenic lines and each line five individual plants for each construct. Different lowercase letters above the bars indicate significant differences at P < 0.05.