FIGURE 10.

SDS::SDS-BARNASE tobacco plants were completely both male and female sterile. (A–C) Large fruits from the WT plant (A) and small fruits from SDS::SDS-BARNASE plants without (B) and with (C) manual pollination with WT pollen grains. Bars = 1 cm. (D) The weight of seeds per self-pollinated and manually pollinated fruit (n = 5), respectively. Numbers indicate examined independent transgenic lines. (E) WT viable pollen grains in red color. (F–H) no (F), all dead (G) and a few viable (H) pollen grains in SDS::SDS-BARNASE plants. Numbers indicate examined independent transgenic lines. Bars = 100 μm.