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. 2016 Feb 1;7:30. doi: 10.3389/fpls.2016.00030

FIGURE 5.

FIGURE 5

SDS::SDS-BARNASE Arabidopsis plants were completely both male and female sterile. (A–C) Primary branches showing normal siliques in wild type (A) and short siliques indicating no developing seeds in SDS::SDS-BARNASE plants without (B) and with (C) pollination. Bars = 1 cm. (D,E) Side view of mature flowers (One sepal was removed, respectively) showing the SDS::SDS-BARNASE flower (E) is similar to the wild type (D) except short filaments. Pollen grains released from WT anthers (D, inset), while no pollen grains from SDS::SDS-BARNASE anthers (E, inset). Bars = 0.5 mm. (F,G) Pollen staining showing the WT anther full of viable pollen grains (F), but no pollen grains from the SDS::SDS-BARNASE anther (G). Bars = 30 μm. (H–J) Dissected individual siliques from primary inflorescences (positions 1–9) were long in wild type (H), but short in SDS::SDS-BARNASE plants (I, without pollination; J, pollinated with WT pollen). Bars = 1 cm.