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. 2016 Feb 1;7:30. doi: 10.3389/fpls.2016.00030

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Copyright © 2016 Huang, Smith, Zhang and Zhao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Formation of female gamete was arrested in SDS::SDS-BARNASE Arabidopsis plants. (A–C) WT ovules showing two separated nuclei (arrows) at the FG3 stage (A), four nuclei (arrows) at the FG4 stage (B), and the central cell, the egg cell, and synergid cells in a mature embryo sac (white dots outlined) at the FG6 stage (C). (D–F) SDS::SDS-BARNASE ovules showing one small nucleus (arrow) at both FG3 (D) and FG4 (E) stages and a small empty embryo sac (white dots outlined) at the FG6 stage (F). Bars = 10 μm. cc, central cell; ec, egg cell; and syn, synergid cells.