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. 2016 Feb 3;7:23. doi: 10.1186/s13287-016-0285-4

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© Wu et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — In vitro differentiation potential of CD146⁺ and CD146^– MSCs. Isolated MSCs were cultured in chondrogenesis or osteogenesis condition medium, and then treated with 10 ng/ml TNFα or 10 % synovial fluid from RA patients. The cells were stained with Safranin O a, b and Alizarin Red S c, d to detect the differentiation potential for chondrogenesis and osteogenesis, respectively. Quantitative data for chondrogenesis and osteogenesis are expressed as mean ± SEM compared with the nondifferentiation-negative control. *P <0.05. RASF synovial fluid from rheumatoid arthritis patients, TNFα tumor necrosis factor alpha