Fig. 5.

HIF-2α promotes EMT through Twist binding to the promoter region of E-cadherin. a Twist1 and Twist2 expressions in response to up-regulation or silencing of HIF-2α in pancreatic cancer cells were detected by Western blot. b ChIP of Twist1 and Twist2 interactions with the E-cadherin promoter. Bands are PCR products targeting P1 and P2 of the E-cadherin promoter. The specific anti-Twist1, anti-Twist2 or control normal mouse IgG was used for immunoprecipitations, whereas genomic DNA was used as the input control