Fig. 3.

Experimental metastasis assay. Mice were injected with test cells via the tail vein as described in Materials and Methods. a. Aperio Precision image analysis on representative lung sections from animals injected with RKO cell derivatives. Twelve mice were used in each group. Left, total tumor area per lung section; right, number of metastases per unit area of lung tissue. P value reflects results of a non-parametric Wilcoxon rank sum test. b. Same analysis for HCT 116 cell derivatives. Ten mice were used in the HCT116^CTRL group and 9 were used in the HCT116^TAGLN-KD group. Statistical analysis as in Panel a. c. Histology of representative tumor sections from mice injected with RKO cell derivatives. d. Same for mice injected with HCT116^CTRL derivatives. HCT116^CTRL-derived tumor is a lung metastasis, HCT116^TAGLN-KD-derived tumor arose near the injection site. e. HCT116-derived tumors stained with anti-transgelin