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. 2016 Feb 4;16:55. doi: 10.1186/s12885-016-2105-8

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© Zhou et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Expression of selected mRNAs determined by qPCR in RKO and DLD-1 cell derivatives. a. Immunoblot analysis of total cell extracts from DLD-1 derivatives stably transfected with empty vector (DLD-1^CTRL) or with transgelin cDNA (DLD-1^TAGLN). b. Real-time RT-PCR analysis for the levels of mRNA in RKO and DLD-1 cells of selected top-ranked differentially expressed genes identified by the cDNA microarray. Gene expression was normalized to GAPDH. Error bars denote standard deviation. All changes were significant (P < 0.05 by Student’s t-test) except for the one result indicated (#)