Skip to main content
. 2016 Feb 3;36(1):e00293. doi: 10.1042/BSR20150290

Figure 1. Identification of miR-34c promoter by luciferase assays.

Figure 1

The luciferase reporter constructs termed as pGL3-883, pGL3-1118 and pGL3-1381 as well as the negative control, pGL3-basic plasmid was each transfected into 293T cells. The dual luciferase assays were performed 24 h after transfection. The relative luciferase activity of each construct was evaluated as relative to the activity of pGL3-basic plasmid, which was arbitrarily set to 1. Transfection of pGL3-1118 and pGL3-1381 produced significantly higher luciferase activities. All luciferase assays were performed in six times (*P<0.05 compared with either pGL3-basic and pGL3-883).