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(A) E2F1 down-regulated the SCF protein expression. Left, western blotting bands; right, grey value analysis (*P<0.05). (B) E2F1 suppressed the SCF mRNA expression. However, none of the previously identified miR-34c targets including Myb, c-Myc, Bcl2, MET and E2F3 were negatively regulated by E2F1 (*P<0.05, **P<0.01).
