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. 2016 Jan 22;72(Pt 2):112–120. doi: 10.1107/S2053230X15024498

Table 2. Crystallization.

Method Vapour diffusion
Plate type Sitting drop
Temperature (K) 293
Protein concentration (mg ml−1) 4
Buffer composition of protein solution 10 mM Tris–HCl pH 7.5, 100 mM NaCl, 2 mM MgCl2
Composition of reservoir solution 8%(w/v) Jeffamine M-2070, 0.17 M glycine, 16.7%(v/v) DMSO, 10 mM urea
Volume and ratio of drop 2 µl; 1:1 ratio
Volume of reservoir (µl) 500