Table 1. Macromolecule-production information.
Source organism | P. aeruginosa POA1 |
DNA source | P. aeruginosa POA1 |
Forward primer† | 5′-CGTATGGATCCATGAAGCAGATGGGTAGCATGC-3′ |
Reverse primer† | 5′-GCATGCGGCCGCTCATACGGCCGAACCTGTCGC-3′ |
Cloning vector | pGEX-6P1 |
Expression vector | pGEX-6P1 |
Expression host | E. coli C41(DE3) |
Complete amino-acid sequence of the construct produced‡ | GPLGSMKQMGSMHPVQVIAVTGGKGGVGKTNVSVNLALALADLGRRVMLLDADLGLANVDVLLGLTPKRTLADVIEGRCELRDVLLLGPGGVRIVPAASGTQSMVHLSPMQHAGLIQAFSDISDNLDVLVVDTAAGIGDSVVSFVRAAQEVLLVVCDEPTSITDAYALIKLLNRDHGMTRFRVLANMAHSPQEGRNLFAKLTKVTDRFLDVALQYVGVIPYDESVRKAVQKQRAVYEAFPRSKASLAFKAVAQKVDSWPLPANPRGHLEFFVERLVQHPATGSAV |
Restriction sites are underlined.
Amino acids from the vector are underlined.