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. 2016 Jan 22;72(Pt 2):135–138. doi: 10.1107/S2053230X16000170

Table 1. Macromolecule-production information.

Source organism P. aeruginosa POA1
DNA source P. aeruginosa POA1
Forward primer 5′-CGTATGGATCCATGAAGCAGATGGGTAGCATGC-3′
Reverse primer 5′-GCATGCGGCCGCTCATACGGCCGAACCTGTCGC-3′
Cloning vector pGEX-6P1
Expression vector pGEX-6P1
Expression host E. coli C41(DE3)
Complete amino-acid sequence of the construct produced GPLGSMKQMGSMHPVQVIAVTGGKGGVGKTNVSVNLALALADLGRRVMLLDADLGLANVDVLLGLTPKRTLADVIEGRCELRDVLLLGPGGVRIVPAASGTQSMVHLSPMQHAGLIQAFSDISDNLDVLVVDTAAGIGDSVVSFVRAAQEVLLVVCDEPTSITDAYALIKLLNRDHGMTRFRVLANMAHSPQEGRNLFAKLTKVTDRFLDVALQYVGVIPYDESVRKAVQKQRAVYEAFPRSKASLAFKAVAQKVDSWPLPANPRGHLEFFVERLVQHPATGSAV

Restriction sites are underlined.

Amino acids from the vector are underlined.