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. 2016 Jan 22;72(Pt 2):139–144. doi: 10.1107/S2053230X16000212

Table 1. Macromolecule-production information.

Source organism M. tuberculosis strain H37Rv
DNA source M. tuberculosis strain H37Rv genome
Forward primer GAAAAAACATATGGGCGGCACCAGCCTGTTCAC
Reverse primer TGGGAATTCTCAGGGGGCTCCGGAGGCT
Cloning vector pET-28a(+) (pLW)
Expression vector pET-28a(+) (pLW)
Expression host E. coli BL21(DE3)
Complete amino-acid sequence of the construct produced MGSSHHHHHHSSGLEVLFQGPHMGTSLFTDRATNQLYVLLSGQLHPVYNLTSARLVLGNPANPATVKSSELSKLPMGQTVGIPGAPYATPVSAGSTSIWTLCDTVARADSTSPVVQTAVIAMPLEIDASIDPLQSHEAVLVSYQGETWIVTTKGRHAIDLTDRALTSSMGIPVTARPTPISEGMFNALPDMGPWQLPPIPAAGAPNSLGLPDDLVIGSVFQIHTDKGPQYYVVLPDGIAQVNATTAAALRATQAHGLVAPPAMVPSLVVRIAERVYPSPLPDEPLKIVSRPQDPALCWSWQRSAGDQSPQSTVLSGRHLPISPSAMNMGIKQIHGTATVYLDGGKFVALQSPDPRYTESMYYIDPQGVRYGVPNAETAKSLGLSSPQNAPWEIVRLLVDGPVLSKDAALLEHDTLPADPSPRKVPAGASGAP