Table 2. Crystallization.

Method	Sitting drop	Sitting drop	Hanging drop	Hanging drop, seeding
Plate type	96 × 3 well	96 × 3 well	24 well	24 well
Temperature (K)	293	293	288	277
Protein concentration (mg ml−1)	10	10	8	5
Buffer composition of protein solution	10 mM Na HEPES pH 7.5, 0.15 M NaCl, 5% glycerol, 4.5 mM TCEP	10 mM Na HEPES pH 7.5, 0.15 M NaCl, 5% glycerol, 4.5 mM TCEP	10 mM Na HEPES pH 7.5, 0.15 M NaCl, 5% glycerol, 4.5 mM TCEP	10 mM Na HEPES pH 7.5, 0.15 M NaCl, 5% glycerol, 4.5 mM TCEP
Composition of reservoir solution	0.1 M Na HEPES pH 7, 1.6 M ammonium sulfate	0.1 M bis-tris propane pH 7, 3.5 M sodium formate	0.1 M Tris–HCl pH 8, 1.4 M ammonium sulfate	0.1 M bis-tris propane pH 7, 1.0 M DL-malic acid
Initial hit	AmSO4 E10	SaltRX C7	AmSO4 E10	SaltRX C9
Volume and ratio of drop	100 nl (1:1 ratio)	100 nl (1:1 ratio)	1 µl (1:1 ratio)	1 µl (1:1 ratio)
Volume of reservoir (µl)	100	100	300	300
Screened using X-rays	No	No	Yes	Yes