Table 2. Observation of dehydrating solutions in open air.

Dehydrating solution	1 h	3 h	Overnight
2 (0.26 M potassium/sodium tartrate, 35% glycerol)†	Clear	Clear	Clear
3 (0.26 M NH4H2PO4, 35% glycerol)†	Clear	Clear	Clear
4 [1.5 M (NH4)2SO4 pH 8.5, 25% glycerol]†	Dried out quickly	Many salt crystals
6 (24% PEG 4K pH 8.5, 0.16 M MgCl2, 20% glycerol)†	Dried out slowly
7 (0.98 M sodium acetate pH 6.5, 30% glycerol)†	Clear	Clear	Clear
8 (21% 2-propanol pH 6.5, 0.14 M sodium citrate, 30% glycerol)†	Clear	Clear	Clear
16 (1.125 M Li2SO4 pH 7.5, 25% glycerol)†	Dried out slowly	Some salt crystals
34 (1.4 M sodium formate pH 4.6, 30% glycerol)†	Clear	Clear	Clear
35 (0.6 M NaH2PO4/K2HPO4 pH 7.5, 25% glycerol)†	Dried out slowly	Some salt crystals
38 (1.26 M sodium citrate pH 7.5, 10% glycerol)†	Clear	Clear	Clear
0.75 M NH4H2PO4 pH 4.5, 25% glycerol	Clear	Clear	Clear
0.75 M ammonium tartrate pH 4.6, 25% glycerol	Clear	Clear	Some salt crystals
26.5% Tacsimate pH 7.0, 25% glycerol	Clear	Clear	Clear
1.875 M sodium formate pH 8.0, 25% glycerol	Clear	Clear	Clear
25% ethanol, 0.1 M sodium citrate, 25% glycerol	Clear	Clear	Clear
15% PEG 8K pH 8.0, 20% glycerol	Dried out slowly
12% PEG 3350, 0.08 M MgCl2, 20% glycerol	Dried out quickly
12% PEG 3350, 0.08 M MgCl2, 20% MPD	Dried out quickly
12% PEG 3350, 0.08 M MgCl2, 20% PEG 400	Dried out quickly
12% PEG 3350, 0.08 M MgCl2, 20% ethylene glycol	Clear	Clear	Clear
0.26 M potassium/sodium tartrate, 25% MPD	Dried out and phase separation
0.26 M potassium/sodium tartrate, 25% ethylene glycol	Clear	Clear	Clear

^†These conditions were selected from the Crystal Screen Cryo kit (Hampton Research) and are numbered accordingly.