FIG. 3.

Histological and immunohistochemical analysis of the bioengineered human pylorus constructs. Alignment of SMCs within bioengineered innervated human pylorus constructs was assessed by hematoxylin and eosin (H&E) staining (200× magnification). H&Es demonstrated compacted circularly aligned SMCs within pylorus constructs after 10 days. Circumferential SMC alignment was further verified by SMA (600× magnification) and smooth muscle specific caldesmon (600× magnification) staining. The presence of differentiated neurons innervating these bioengineered constructs was verified through positive staining for pan-neuronal marker, βIII-tubulin, after 10 days (600× magnification). All scale bars = 100 μm. Color images available online at www.liebertpub.com/tea