Figure 4.
Effect of CE on HEWL amyloid fibrils when added after the nucleation step. CE (with 1 mg/ml final concentration) was added to HEWL samples incubated for 36 h, at pH 2.5 and 57 °C. ThT fluorescence changes were then monitored over 48 h incubation in the same condition (□). HEWL in the absence of CE is indicated by (▪). Protein concentration was 2 mg/ml.