. Author manuscript; available in PMC: 2016 Oct 27.

Published in final edited form as: Neurochem Res. 2015 Oct 27;40(10):2131–2142. doi: 10.1007/s11064-015-1705-z

Table V. Determination of agonist activity of A-84543, H-11MNH and nicotine at rat α3β4 nAChRs by measuring stimulation of ⁸⁶Rb⁺ efflux from transfected cells.

The concentration-dependent activation of nAChR function of α3β4 transfected cells by the compounds was measured as described previously [35]. The EC₅₀ values and Relative E_max are the mean ± SEM of 3 independent experiments.

Agonist Activity
Compound	EC₅₀ (μM)	^* Relative E_max
A-84543	160 ± 40	37.5 ± 6
H-11MNH	24 ± 1	83.3 ± 3
Nicotine	32 ± 2	100 ± 8

% of the stimulation by 100 μM nicotine

Table V. Determination of agonist activity of A-84543, H-11MNH and nicotine at rat α3β4 nAChRs by measuring stimulation of 86Rb+ efflux from transfected cells.

Table V. Determination of agonist activity of A-84543, H-11MNH and nicotine at rat α3β4 nAChRs by measuring stimulation of ⁸⁶Rb⁺ efflux from transfected cells.