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. 2015 Dec 16;35(3):356–368. doi: 10.15252/embj.201592116

Figure 3. Tcf7l1 null ES cells do not require CH to initiate endoderm differentiation programme.

Figure 3

  1. Assay of FoxA2 mRNA by RT–PCR in Tcf7l1 null cells on day 1 to 4 of differentiation without CH. Average and SD of three independent experiments.
  2. Gene expression analysis of endodermal associated genes by RT–PCR in control wild‐type and Tcf7l1 null cells on day 4 of differentiation in the presence and absence of 3 μM CH. Average and SD of three independent experiments.
  3. FoxA2 (green) and Oct4 (red) immunostaining of Tcf7l1 null ES cells on day 4 of differentiation in the presence or absence of 3 μM CH. DAPI staining in blue. Scale bars, 200 μm.
  4. Assay of CXCR4, Hhex and Sox17 mRNA by RT–PCR in control wild‐type and Tcf7l1 null cells on day 6 of differentiation in the presence and absence of 3 μM CH. Average and SD of three independent experiments.