FIGURE 8. COX2 expression is induced by WTLM and ΔhlyLM and is independentofTLR2.
Resident mouse peritoneal macrophages isolated from ICR (A) and TLR2−/−and TLR2+/+mice (B) were infected (m.o.i. 25) with WTLM or ΔhlyLM. After a 1-h incubation with L. monocytogenes, the macrophages were washed and then incubated in medium containing gentamicin. Expression of COX2 and COX1 was determined by Westernblotting of macrophage lysates prepared at the times indicated after addition of L. monocytogenes. Uninf, uninfected.