Skip to main content
. 2015 May 4;168(3):930–937. doi: 10.1104/pp.15.00005

Figure 2.

Figure 2.

Validation of the isolated dwarfing gene. A, Allelism tests between SAO and N669422 (a transferred DNA insertional mutant of GA5). Means ± se of plant height when the first flower opens (PHa) and plant height toward the end of growth (PHb) of indicated genotypes are shown. Letters above bars indicate statistical significance of pairwise differences within each group, as examined by Tukey’s post hoc tests. B, Validation of transformation of the functional GA5 copy from Col-0 into ga5-184 background using a cleaved-amplified polymorphic sequence (CAPS) marker in both genomic and transcriptional levels (for more details, see “Materials and Methods”). Templates for PCR are indicated on the left, whereas genotypes are shown above. gDNA, Genomic DNA; cDNA, complementary DNA. C, Morphology of plants when the first flower opens in different genetic backgrounds as indicated. Plants were germinated and grown under the same conditions, and photos were taken at different dates due to different flowering times. D, Measurements of PHa and PHb of indicated genetic backgrounds. Letters above bars indicate statistical significance of pairwise differences within each group, as examined by Tukey’s post hoc tests.