Figure 1.

BAK1 overexpression causes necrosis and growth defects in Arabidopsis. A, Photographs of two transgenic T1 plants overexpressing BAK1 under the control of the 2× 35S promoter from Cauliflower mosaic virus (BAK1-ox) exhibiting a strongly stunted phenotype in contrast to wild-type (WT) Columbia-0 (Col-0) plants after 6 weeks of growth. B, Western-blot analysis of whole extract from the two presented lines with anti-BAK1 antibodies. The arrowhead indicates full-length BAK1, and the asterisks signal the presence of two additional bands. Protein loading control by Ponceau S is shown. C, Of the 60 independent T1 lines isolated from 10 independent selections, about 50% had a milder phenotype and developed symptoms at a later time point.