Figure 5.

Involvement of AtHXK1-dependent Glc signaling during BR regulation of hypocotyl elongation growth. Quantification of hypocotyl elongation in 7-d-old etiolated seedlings of the wild type (Ler) and AtHXK1-dependent Glc sensor mutant gin2-1 growing on Glc-free (0%) medium or increasing concentrations of Glc (1%, 3%, and 5% [w/v]) containing one-half-strength MS medium supplemented without or with increasing BR concentrations (10 nm, 100 nm, and 1 µm). The gin2-1 mutant showed less response to BR in terms of hypocotyl elongation growth compared with the wild type. Values represent the average from two biological replicates, each having 30 seedlings, and error bar represents se (Student’s t test, P < 0.001; *, control versus treatment; and **, wild type versus mutant).