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. 2015 Jun 1;168(3):1091–1105. doi: 10.1104/pp.15.00495

Figure 9.

Figure 9.

Involvement of TIR1/AFB mediated auxin perception during Glc-BR for regulation of hypocotyl elongation in etiolated seedlings. Quantification of hypocotyl elongation growth in the 7-d-old etiolated wild type (Col-0 and Wassilewskija), the auxin-signaling mutant tir1 (A), and tir1afb1-3afb2-3afb3-4 quadruple mutant seedlings (B) growing on one-half-strength MS medium supplemented with independent as well as combined treatments of Glc and BR. The tir1 mutants displayed wild-type-like sensitivity for Glc and higher concentrations of BR treatments. However, the tir1afb1-3afb2-3afb3-4 quadruple mutant showed resistance for Glc and BR compared with the wild type in terms of etiolated hypocotyl elongation growth. These results indicate the involvement of TIR1/AFB-dependent auxin perception during BR-Glc cross talk. Values represent the average from two independent biological replicates, each having 20 seedlings, and error bars represent se. (Student’s t test, P < 0.001; *, control versus treatment; and **, wild type versus mutant).