Table II. Relative fluxes across the nine oilseed rape entries.
c, Compartment-specific enzyme isoform or flux (cytosol); p, compartment-specific enzyme isoform or flux (plastid).
Parameter | BCS1875 | CR2277 | CR3231 | CR3135 | BCS1859 | DGAT | OSR Reference Line | CR2186 | CR3217 | Average |
---|---|---|---|---|---|---|---|---|---|---|
% | ||||||||||
Carbon conversion efficiencya | 84 | 86 | 86 | 83 | 84 | 80 | 80 | 80 | 79 | 82 ± 03 |
Relative Rubisco fluxb | 68 | 59 | 32 | 62 | 59 | 51 | 42 | 51 | 46 | 52 ± 11 |
PEP-consuming reactions relative to flux into plastidic fatty acid synthesis (vFASp) | ||||||||||
PKp (vPKp) | 62 | 54 | 71 | 72 | 74 | 74 | 72 | 67 | 71 | 69 ± 7 |
Cytosolic pyruvate kinase (PKc; vPKc) | 28 | 42 | 27 | 20 | 19 | 18 | 21 | 24 | 26 | 25 ± 7 |
PEPC (vPEPC) | 7 | 6 | 4 | 6 | 6 | 5 | 6 | 6 | 6 | 6 ± 1 |
NADPH production by OPPP relative to NADPH consumption by plastidic fatty acid synthesis (vFASp)c | ||||||||||
NADPH by plastidic OPPP | 2 | 0 | 0 | 5 | 3 | 9 | 7 | 5 | 2 | 4 ± 3 |
NADPH by plastidic and cytosolic OPPP | 47 | 11 | 0 | 50 | 37 | 39 | 25 | 62 | 51 | 36 ± 20 |
Percentage of total carbon uptake being stored in biomass. b3-Phosphoglyceric acid (3-PGA) generated by Rubisco. cNADPH production: 2 × OPPP flux (two NADPHs per molecule of Glc-6-P oxidized). NADPH consumption is based on fatty acid biosynthetic flux (vFASp), considering the fatty acid chain length distribution and distinguishing cytosolic fatty acid synthesis. Per elongation cycle of an acyl chain, one NADPH and one NADH are required for plastidic fatty acid synthase and two NADPHs are required for cytosolic elongase.