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. 2015 May 5;168(3):828–848. doi: 10.1104/pp.15.00385

Table II. Relative fluxes across the nine oilseed rape entries.

c, Compartment-specific enzyme isoform or flux (cytosol); p, compartment-specific enzyme isoform or flux (plastid).

Parameter BCS1875 CR2277 CR3231 CR3135 BCS1859 DGAT OSR Reference Line CR2186 CR3217 Average
%
Carbon conversion efficiencya 84 86 86 83 84 80 80 80 79 82 ± 03
Relative Rubisco fluxb 68 59 32 62 59 51 42 51 46 52 ± 11
PEP-consuming reactions relative to flux into plastidic fatty acid synthesis (vFASp)
PKp (vPKp) 62 54 71 72 74 74 72 67 71 69 ± 7
 Cytosolic pyruvate kinase (PKc; vPKc) 28 42 27 20 19 18 21 24 26 25 ± 7
PEPC (vPEPC) 7 6 4 6 6 5 6 6 6 6 ± 1
NADPH production by OPPP relative to NADPH consumption by plastidic fatty acid synthesis (vFASp)c
 NADPH by plastidic OPPP 2 0 0 5 3 9 7 5 2 4 ± 3
 NADPH by plastidic and cytosolic OPPP 47 11 0 50 37 39 25 62 51 36 ± 20
a

Percentage of total carbon uptake being stored in biomass.  b3-Phosphoglyceric acid (3-PGA) generated by Rubisco.  cNADPH production: 2 × OPPP flux (two NADPHs per molecule of Glc-6-P oxidized). NADPH consumption is based on fatty acid biosynthetic flux (vFASp), considering the fatty acid chain length distribution and distinguishing cytosolic fatty acid synthesis. Per elongation cycle of an acyl chain, one NADPH and one NADH are required for plastidic fatty acid synthase and two NADPHs are required for cytosolic elongase.