Characterization of the tang1 mutant grown in the light. A, Root lengths of Col-0, tang1-1, and tang1-2 seedlings grown on one-half-strength MS medium supplemented with 3% (w/v) Glc (G) or 3% (w/v) mannitol (M) at the indicated days. Data represent means ± se (n > 20) of two independent biological replicates. B, Chlorophyll levels in 10-d-old Col-0, tang1-1, and tang1-2 seedlings grown on one-half-strength MS medium or one-half-strength MS medium supplemented with 2% (w/v) Glc. Data represent means ± se of three independent biological replicates. *, P < 0.05; **, P < 0.01 compared with related Col-0 (one-way ANOVA); F.W., fresh weight. C, Starch contents in 20-d-old wild-type and tang1-1 seedlings grown on medium with 3% (w/v) mannitol or 3% (w/v) Glc. Data represent means ± se of three independent biological replicates. **, P < 0.01 compared with the 3% (w/v) Glc Col-0 sample (one-way ANOVA). D, Anthocyanin levels in 20-d-old wild-type and tang1-1 seedlings grown on medium with 3% (w/v) mannitol or 3% (w/v) Glc. Data represent means ± se of three independent biological replicates. **, P < 0.01 compared with the 3% (w/v) Glc Col-0 sample (one-way ANOVA).