Figure 4.

Metabolomics analysis. A to D, Metabolites in rosette leaves of wild-type (Columbia-0 [Col-0]; black), cyp71a12 (green), cyp71a13 (red), and cyp71a12 cyp71a13 (blue) plants, 24 h after detachment and spraying with AgNO₃ (A), 22 h after detachment and 2 h of UV irradiation (B), untreated (C), or 24 h after detachment and spraying with water (D). Characterized metabolites for which significant differences in cyp71a12, cyp71a13, or cyp71a12 cyp71a13 in comparison with the wild type were observed. The full data set is presented in Supplemental Table S1. Error bars indicate se (n = 9–11, except n = 20 for cyp71a12 cyp71a13 in A and D). DHCA, Dihydrocamalexic acid; HC MalonylHex, hydroxycamalexin malonylhexoside; ICOOGlc, Glc ester of ICOOH; 6-OH-ICOOH, 6-hydroxyindole-3-carboxylic acid; 6-GlcO-ICOOH, 6-hydroxyindole-3-carboxylic acid 6-O-β-d-glucoside. E, Relative quantification of Trp in Col-0 (black) and cyp79b2 cyp79b3 (violet) in the data sets denoted. Significance analysis of differences between the wild type and mutant was performed by two-tailed Student’s t test (*, P ≤ 0.05; **, P ≤ 0.01; and ***, P ≤ 0.001).