Pruned subtree of CslE, CslJ, and CslH clade and codeml branch-site tests. Branch lengths are proportional to substitutions per site. Postduplication branches with sites that codeml has identified as under significant positive selection are colored blue. Predicted gene duplication events are annotated as red dots. The star indicates the branch whose sequence divergence was greater than 75%. ω denotes dN:dS values. Sites with no differences in nonsynonymous substitutions yielded the PAML dN:dS ceiling ratio of 999. The overall dN:dS values for the CslE, CslJ, and CslH clades are 0.23, 0.4, and 0.25, respectively (Fig. 3).