Figure 1.

Seed germination, polysome profiling, and changes in abundance of mRNAs in the transcriptome and translatome. A, Germination of Arabidopsis seeds on water at 25°C in darkness during 8 d: 1, freshly harvested seeds (dormant seeds); 2, dormant seeds after-ripened for 3 weeks at 20°C at 56% relative humidity; 3, dormant seeds after-ripened for 4 weeks at 20°C at 56% relative humidity (nondormant seeds). Means ± sd of triplicate experiments are shown. B and C, Suc density gradient profiles of RNA (A₂₅₄) from Arabidopsis seeds. Peaks 1 and 2 correspond to 60S ribosomal subunits and to 80S monosomes, respectively. B, Absorbance profiles of mRNA obtained from dormant dry seeds and during their imbibition (3, 6, 16, and 24 h) at 25°C. C, Comparison of absorbance profiles obtained with dormant (D; black solid lines) and nondormant (ND; red dotted lines) seeds after 16 and 24 h of imbibition at 25°C. D and E, Analysis of microarray data. Venn diagrams show the distribution of mRNAs presenting abundance variations (after BH correction with P < 0.05 and log₂ ratio above 0.5 or under −0.5) in total RNA (T; D) and in polysomal RNA (P; E) after 16 and 24 h of imbibition at 25°C. Numbers in parentheses correspond to the sum of all transcripts in a category.