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. Author manuscript; available in PMC: 2016 Feb 4.
Published in final edited form as: Science. 2006 Aug 25;313(5790):1137–1140. doi: 10.1126/science.1128294

Fig. 2.

Fig. 2

Effect of PBA treatment on markers of ER stress in the liver tissue of ob/ob mice. (A) Phosphorylation of PERK (Thr980) and IRE-1α in liver tissues of PBA- or vehicle-treated ob/ob mice and lean controls. (B) Liver tissue total JNK activity, JNK protein levels, serine phosphorylation of IRS-1 (Ser-307), and total JNK and IRS-1 protein levels in the same group of mice. (C) Insulin-stimulated insulin receptor (IR), insulin receptor substrate 1 (IRS-1), insulin receptor substrate 2 (IRS-2), and (D) Akt (Ser-473) phosphorylation in liver tissues of PBA- and vehicle-treated lean and ob/ob mice upon insulin (2 IU/kg) infusion through the portal vein. The graphs on the right of each blot show the quantitation of phosphorylation for each protein. Data are presented as the means ± SEM, and asterisks indicate statistical significance determined by student’s t test (*P < 0.05, **P < 0.001).