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(A, B) WT and A25T Cys C amino acid sequence was analyzed by SignalP 4.1 software and the resultant predicted cleavage sites, designated as site 1 and site 2, are shown. (C) In WT Cys C, site 1 cleavage occurs between Ala20 and Val21, site 2 cleavage occurs between Gly26 and Ser 27. The Ala residue in the A25T mutation is enlarged and colored green.
