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. 2015 Oct 24;6(33):34143–34157. doi: 10.18632/oncotarget.6219

Figure 9. Increased NF-κB activation during LPS induced aging.

Figure 9

Western blot analyses of liver nuclear and cytosolic A. NF-κB, COX-2, and iNOS levels were performed using the nuclear and cytosolic proteins of LPS treated old rats. Results are representative of three independent experiments. B. Nuclear extracts were prepared from the liver of young and old LPS treated rats. Immunoprecipitated FoxO6 was determined to be physically associated with NF-κB by Western blotting. C. ROS generation in LPS treated old rats was assessed in liver homogenates using the DCFHDA method. Results are presented as means ± SEs of 4 rats. Young = 6 months old; Old = 24 months old. Results of one factor ANOVA: #p < 0.05 vs. Young rats; *p < 0.05 vs. LPS treated age-matched rats. D. Possible mechanism of the effect of FoxO6 on LPS stimulated aging. ROS, reactive oxygen species; FoxO6, Forkhead transcription factor O6; NF-κB, Nuclear transcription factor κB; Pak1, P21-activated kinase.