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. 2015 Oct 6;6(33):34228–34244. doi: 10.18632/oncotarget.5349

Figure 6. Barrett's esophagus and esophageal adenocarcinoma cells in organotypic reconstruct cultures.

Figure 6

A. OE33 cells had increased Alcian Blue staining upon A83-01 treatment (arrows). OE33 cells were also positive for the columnar marker cytokeratin 19 (keratin 19). B. CPB and FLO-1 cells, which have a mesenchymal phenotype, express no E-cadherin, but OE33 showed positive E-cadherin staining by immunofluorescence. Anti-keratin 8 antibody showed that the signal increased in CPB following stimulation with Activin A or treatment with A83-01. Keratin 8 signal was weaker in FLO-1 cells in the presence of A83-01.