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. 2015 Sep 10;6(33):34245–34257. doi: 10.18632/oncotarget.5196

Figure 4. JAK2 down-modulation by siRNAs prevents chemokine-induced adhesion and LFA-1 affinity triggering.

Figure 4

A. Western blot of total cell lysates of B-lymphocytes not treated (Control), nucleoporated with a pool of 4 scrambled or JAK2-specific siRNAS and kept in culture for 24 h or 48 h. Shown is protein content compared to actin; one representative experiment of three. B. Quantification of immunoreactive bands of n = 3 independent experiments. The y-axis represents the relative JAK2/actin protein ratio normalized to the Control value. Mean ± SD. *P < 0.05; **P < 0.01, versus scrambled siRNAs. Static adhesion to ICAM-1 C or VCAM-1 D. Cells were treated as in (A) and after 48 h were collected and stimulated with CXCL12 0.5 μM for 120 sec. Mean ± SD. *P < 0.001, versus scrambled siRNAs; data are average of n = 3 independent experiments in triplicate. E. KIM127 or F 327A staining: cells were treated as in (A) and after 48 h were collected and stimulated with CXCL12 0.5 μM for 120 sec. Mean ± SD. *P < 0.01, versus scrambled siRNAs; data are average of n = 3 independent experiments.