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. 2015 Oct 5;6(33):34342–34357. doi: 10.18632/oncotarget.5326

Figure 3. Effect of autoubiquitination of LNX2 on Numb ubiquitination.

Figure 3

In vitro ubiquitination assays were performed using E1, E2 (UbcH5b), unmodified FL-LNX2 or MBP beads immobilized autoubiquitinated FL-LNX2, GST-Numb, Ubiquitin and ATP at different time intervals, as indicated. +ve control-Positive control where unmodified or non-ubiquitinated FL-LNX2 was used for the ubiquitination assay. Shown are the immunoblots using anti-Numb antibodies for unmodified FL-LNX2 A. and autoubiquitinated FL-LNX2 B. The blot in (B) was stripped and again immunoblotted with anti-LNX2 antibody C.