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In vitro ubiquitination assays were performed for the Zn-RING-Zn domain in the presence (+) or absence (−) of each of E1, E2 (UbcH5b), Zn-RING-Zn domain, Ubiquitin and ATP, respectively. Shown are the immunoblots for the autoubiquitination of Zn-RING-Zn domain using anti-Ubiquitin (left) and anti-LNX2 (right) antibodies.
