Skip to main content
. 2015 Oct 5;6(33):34342–34357. doi: 10.18632/oncotarget.5326

Figure 7. Dimer-disrupted mutant Lys109Ala and its effect on activity of Zn-RING-Zn domain.

Figure 7

A. WT Zn-RING-Zn and point mutants of Zn-RING-Zn, Lys109Ala and Asp45Ala were each separately used for gel-filtration chromatography. Their respective elution profiles were overlaid and compared. The molecular mass standard is shown in blue. B. In vitro ubiquitination assays were performed using E1, E2 (UbcH5b), Ubiquitin, ATP, WT-Zn-RING-Zn or Lys109Ala mutant Zn-RING-Zn with increasing concentrations, as indicated. Shown is the immunoblot using the anti-Ubiquitin antibody.