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. 2015 Sep 19;6(33):34389–34401. doi: 10.18632/oncotarget.5360

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Copyright: © 2015 Shapiro et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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A. Ls174T CRC cell clones stably overexpressing CLU were isolated after transfection with CLU cDNA (CLU Cl1–3). The levels of CLU in selected individual clones were determined by western blotting with antibody against CLU. B. Immunofluorescence staining for CLU in a CLU-overexpressing CRC cell clone (CLU Cl1) and in control (pcDNA3)-transfected CRC cells (Con1) with anti-CLU antibody and with DAPI for nuclei staining. C. An artificial wound was introduced into a monolayer of cells, and cell motility into the wound area (after 24 h) was followed in three different wounds for each cell clone (as indicated) and expressed as percent wound closure compared to time 0 h when the wound was introduced. **p < 0.01. Error bars: ±S.D.