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. 2015 Sep 30;6(33):34475–34493. doi: 10.18632/oncotarget.5922

Figure 1. Morphology and differentiation of ASCs isolated from subcutaneous and visceral adipose tissue.

Figure 1

A. Immunofluorescence staining. Visceral ASCs (ASCvis) and subcutaneous ASCs (ASCsub) were fixed and stained for α-tubulin, vimentin, pericentrin and DNA. Examples are shown. Scale bar: 20 μm. B. Immunofluorescence staining of cell surface markers in ASCs. Scale bar: 10 μm. C. Multilineage differentiation capability of ASCs. ASCs were incubated with corresponding differentiation medium for 14 or 21 days. Neuronal differentiation (14 days) was verified with lineage-specific staining of Tuj1 and DCX. Adipogenic differentiation (14 days) was assessed by oil red O staining displaying lipid vesicle formation. Osteogenic differentiation (21 days) was examined by Alizarin Red S staining indicating calcium deposits. D. Quantification of the differentiation potential of visceral ASCs by analyzing lineage-specific characteristic marker (n = 300 cells for each condition). The results are based on three independent experiments with ASCs obtained from three different donors and presented as mean ± SEM (n = 3).