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. 2015 Sep 30;6(33):34475–34493. doi: 10.18632/oncotarget.5922

Figure 2. ASCs interact with cancer cells directly and indirectly.

Figure 2

A. The cytokine/chemokine array assay. The factors were measured in the supernatants of visceral ASCs (ASCvis) and subcutaneous ASCs (ASCsub) cultured for 3 days by using a human cytokine antibody array. The six most prominent chemokines/chemokines are demonstrated. The results, relative to the positive control provided by the array, are based on three independent experiments with ASCs obtained from three different donors and presented as mean ± SEM. B. Evaluation of membrane protrusion of ASCs toward MCF-7, MDA-MB-231 and MCF-10A cells. The results are based on three independent experiments with ASCs from three different donors and shown as mean ± SEM. C. Representatives of ASCs homing to breast cancer cell lines. Red arrows indicate membrane protrusions of ASCs toward breast cancer cells. Normal mammary epithelial MCF-10A cells served as negative control. Scale bar: 250 μm. D. Immunofluorescence staining of the migration front between ASCs and MCF-7 or MDA-MB-231 cells. Both cell types were stained for p-FAK, phalloidin, acetylated α-tubulin and DNA. White arrows depict the connections stabilized by acetylated α-tubulin between ASCs and breast cancer cells. Scale bar: 25 μm.