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. 2015 Sep 30;6(33):34475–34493. doi: 10.18632/oncotarget.5922

Figure 3. MCF-7 cells proliferate strongly upon direct co-culturing with ASCs, associated with increased mRNA expression of BCL6, IL-6 and IL-8.

Figure 3

A.-C. Cell viability assay. MCF-10A A., MCF-7 B. and MDA-MB-231 C., in the presence or absence of 20% subcutaneous ASCs (ASCsub) or visceral ASCs (ASCvis), were seeded in 96-well plates. Cell viability was measured via CellTiter-Blue® assay. The results are based on three independent experiments with ASCs from three different donors and presented as mean ± SEM. *p < 0.05, **p < 0.01. D. MCF-7 cells incubated with ASCs for 96 h were stained for phospho-histone H3 (pHH3, S10), a mitotic marker, E-cadherin, CD90 as ASC marker and DNA. Non-treated MCF-7 cells were taken as control. Representatives are shown. Scale bar: 25 μm. E. Cell number of Ruby-H2B MCF-7 cells co-cultured with 20% visceral ASCs or subcutaneous ASCs, evaluated by flow cytometry. The results are based on three independent experiments with ASCs from three different donors and shown as from three different donors and shown as mean ± SEM (n = 3). *p < 0.05, **p < 0.01. F. The gene expression of Ruby-H2B MCF-7 cells cultured alone or co-cultured with ASCs. The results are presented as mean ± SD and the values represent absolute mRNA levels relative to the standard curve. *p < 0.05, **p < 0.01, ***p < 0.001.