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. 2015 Sep 30;6(33):34475–34493. doi: 10.18632/oncotarget.5922

Figure 7. EMT-like MCF-7 cells are resistant to BI compounds.

Figure 7

A.-C. Cell viability assay. MCF-7 cells A., MCF-7 cells incubated with visceral ASCs for 14 days B. and visceral ASCs alone C. were seeded in 96-well plates and treated with Plk1 inhibitors BI 2536 (50 nM), BI 6727 (50 nM) or Poloxin (40 μM) and their viability was measured at indicated time points via CellTiter-Blue® assay. DMSO treated cells served as vehicle control. The results are obtained from three independent experiments with ASCs obtained from three different donors and presented as mean ± SEM (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001. D. The treated cells were stained for pHH3 (S10), vimentin and DNA. The representatives are shown (upper panel: MCF-7 control cells; lower panel: MCF-7 cells co-cultured with visceral ASCs). Scale bar: 25 μm. E. Quantification of positive pHH3 (S10) staining in MCF-7 control cells or in mesenchymal-like MCF-7 cells treated with BI compounds for 48 h. The results are from three independent experiments with ASCs from three different donors and presented as mean ± SD (n = 3). *p < 0.05, **p < 0.01.