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. 2015 Sep 30;6(33):34510–34524. doi: 10.18632/oncotarget.5923

Figure 5. RASSF8 downregulates VEGF-C expression in ESCC cells.

Figure 5

A., RASSF8 protein levels are inversely correlated with VEGF-C in clinical ESCC tissues (n = 23, p = 0.014, R = 0.503). Left: Images of two representative cases. Right: Correlation analyses of RASSF8 and VEGF-C protein expression in 23 human ESCC tissues. Bars = 100 μm. B., ELISA of VEGF-C protein levels in the supernatants of the indicated cells. C., Immunohistochemical staining detection of VEGF-C protein levels in footpad tumors. Bars = 100 μm. D., Western blots of phospho-AKT (p-AKT, Ser 473) and phospho-ERK (p-ERK, T202/Y204) protein levels in TSN-treated human lymphatic endothelial cells (HLECs). E., Western blot analysis of phosphor-AKT (Ser374) and phosphor-ERK (T202/Y204) protein expression in HLECs cultured in TSNs derived from RASSF8 knockdown EC109 cells treated with U0126 or LY294002. F., G., Representative images and quantifications of matrigel tube formation (Bars = 500 μm) and transwell migration (Bars = 100 μm) assays, respectively, of HLECs cultured with TSN from the indicated cell. Error bars indicate the mean±SEM of three independent experiments. *p < 0.05.